Current Research Projects
Engineering Adeno-associated viral vectors for targeting specific subsets of cells in the retina and using these AAV varients for therapeutic gene delivery in the context of retinal degenerations
Methodology
- Directed evolution of AAV capsids, qPCR, rAAV production
- Intraoular injections in rodents (rats, mice)
- Flow cytometry, immunohistochemistry, electroretinography (ERGs)
Selected Publications
- Dalkara D. / Kolstad KD, Guerin K, Visel M, Hoffmann N, Schaffer DV, Flannery JG
Changes in adeno-associated virus-mediated gene delivery in retinal degeneration.
Human Gene Therapy, 2010 May;21(5):571-8. - Klimczak RR, Koerber JT, Dalkara D, Flannery JG, Schaffer DV.
A novel adeno-associated viral variant for efficient and selective intravitreal transduction of rat Müller cells.
PLoS One., 2009 Oct 14;4(10):e7467. - Dalkara D, Kolstad KD, Caporale N, Visel M, Klimczak RR, Schaffer DV, Flannery JG.
Inner limiting membrane barriers to AAV-mediated retinal transduction from the vitreous.
Molecular Therapy, 2009 Dec;17(12):2096-102. Epub 2009 Aug 11. - Koerber JT, Klimczak R, Jang JH, Dalkara D, Flannery JG, Schaffer DV.
Molecular evolution of adeno-associated virus for enhanced glial gene delivery.
Molecular Therapy, 2009 Dec;17(12):2088-95. Epub 2009 Aug 11. - Courtête J, Sibler AP, Zeder-Lutz G, Dalkara D, Oulad-Abdelghani M, Zuber G, Weiss E.
Suppression of cervical carcinoma cell growth by intracytoplasmic codelivery of anti-oncoprotein E6 antibody and small interfering RNA.
Mol Cancer Ther., 2007 Jun;6(6):1728-35.
Deniz Dalkara |
Research Group
The Flannery laboratory
Research Groups
People