Arpad Palfi

Current Research Projects

• Suppression and replacement strategies towards development of gene therapeutic approaches for retinal degenerations
• Role and mechanism of action of microRNAs in the normal and degenerating retina

Methodology

Histology, immunohystochemistry, PAGE/Western-blot, in situ hybridization, qRT-PCR, microarray, cloning, cell culture, FACS, RNAi, microRNA, gene transfer, subretinal injection, AAV

Selected Publications

1. Loscher CJ, Hokamp K, Wilson JH, Li T, Humphries P, Farrar GJ, Palfi A.
   A common microRNA signature in mouse models of retinal degeneration.
   Exp Eye Res. 2008 Dec;87(6):529-34. Epub 2008 Sep 13. PMID: 1883487
   
2. Carol Loscher, Karsten Hokamp, Paul F. Kenna, Alasdair C. Ivens, Peter Humphries, Arpad Palfi and G. Jane Farrar.
   Altered retinal microRNA expression profile in mouse model of retinitis pigmentosa.
   Genome Biol. 2007 Nov 22;8(11):R248 PMID: 18034880
   
3. O'Reilly M, Palfi A, Chadderton N, Millington-Ward S, Ader M, Cronin T, Tuohy T, Auricchio A, Hildinger M, Tivnan A, McNally N, Humphries MM, Kiang AS, Humphries P, Kenna PF, Farrar GJ.
   RNA interference-mediated suppression and replacement of human rhodopsin in vivo.
   Am J Hum Genet. 2007 Jul;81(1):127-35. Epub 2007 May 23. PMID: 17564969
   
4. Kennan A, Aherne A, Palfi A, Humphries M, McKee A, Stitt A, Simpson DA, Demtroder K, Orntoft T, Ayuso C, Kenna PF, Farrar GJ, Humphries P.
   Identification of an IMPDH1 mutation in autosomal dominant retinitis pigmentosa (RP10) revealed following comparative microarray analysis of transcripts derived from retinas of wild-type and Rho(-/-) mice.
   Hum Mol Genet 2002 Mar 1; 11(5):547-57, PMID: 11875049
   
5. Millington-Ward S, Oneill B, Tuohy G, Al-Jandal N, Kiang AS, Kenna PF, Palfi A, Hayden P, Mansergh F, Kennan A, Humphries P, Farrar GJ.
   Strategems in vitro for gene therapies directed to dominant mutations.
   Hum Mol Genet 1997, 9:1415-1426, PMID: 9285777