Stefanie Hauck

Current Research Projects

Identification of neuroprotective factors which prolong survival of retinal neurons, especially photoreceptors in the context of inherited or age-related retinal degenerations. We focus on intrinsic factors which are secreted or presented by retinal Mueller Glial cells and investigate the relevant signal transduction pathways for neuron-glia communication. Additionally, we study pathomechanisms of autoimmune Uveitis in a spontaneous animal model (horse; ERU).

Methodology

• Quantitative proteomics, both gel-based (DIGE technology) as well as gel-free (LC-MSMS) methods
• Primary cell assays (photoreceptors, Mueller glia, RPE)
• Protein interaction studies (TAP pull-downs, Yeast-two-hybrid screens, Biacore)
• Mass spectrometry (MALDI-MSMS: AB4700, LC-MSMS: OrbiTrap)
• Histology

Selected Publications

1. SM Hauck, CJ Gloeckner, ME Harley, S Schoeffmann, K Boldt, P Ekström and M Ueffing
   Identification of paracrine neuroprotective candidate proteins by a functional assay-driven proteomics approach
   Mol Cell Proteomics. 2008 Jul;7(7):1349-61
2. CA Deeg, AJ Raith, B Amann, JW Crabb, SR Thurau, SM Hauck, M Ueffing, G Wildner and M Stangassinger
   CRALBP is a highly prevalent autoantigen for human autoimmune uveitis
   Clin Dev Immunol, 2007:39245
3. F Paquet-Durand, J Silva, LE Johnson, T Talukdar, S Azadi, T van Veen, M Ueffing, SM Hauck and P Ekström
   Excessive activation of poly (ADP-ribose) polymerase (PARP) contributes to rd1 photoreceptor degeneration
   J Neurosci, 2007 Sep 19;27(38):10311-9
4. M Swiatek-De Lange, A Stampfl, SM Hauck, H Zischka, CJ Gloeckner, CA Deeg, M Ueffing
   Membrane-initiated effects of progesterone on calcium dependent signaling and activation of VEGF gene expression in retinal glial cells
   Glia. 2007 Aug 1;55(10):1061-73.
5. CA Deeg, SM Hauck, B Amann, E Kremmer, M Stangassinger and M Ueffing
   Major retinal autoantigens remain stably expressed during all stages of spontaneous uveitis
   Mol Immunol. 2007, Jul;44(13):3291-6
6. SM Hauck, S Schoeffmann, B Amann, M Stangassinger, H Gerhards, M Ueffing and CA Deeg
   Retinal mueller glial cells trigger the hallmark inflammatory process in autoimmune uveitis
   J. Prot. Res. 2007, Jun;6(6):2121-31
7. CA Deeg, F Altmann, SM Hauck, S Schoeffmann, B Amann, M Stangassinger and M Ueffing
   Downregulation of PEDF in uveitic lesions associates with focal VEGF expression and breakdown of the blood retinal barrier
   Proteomics. 2007, May;7(9):1540-8
8. CA Deeg, D Pompetzki, AJ Raith, SM Hauck, B Amann, S Suppmann, TW Gobel, U Olazabal, H Gerhards, S Reese, M Stangassinger, B Kaspers and M Ueffing
   Identification and functional validation of novel autoantigens in equine uveitis.
   Mol Cell Proteomics. 2006 Aug; 5(8):1462-70
9. SM Hauck, N Kinkl, CA Deeg, M Swiatek-de Lange, S Schoeffmann and M Ueffing
   GDNF Family Ligands Trigger Indirect Neuroprotective Signaling in Retinal Glial Cells
   Mol Cell Biol., 2006 Apr; 26(7):2746-57
10. CS Alge, SM Hauck, SG Priglinger, A Kampik and M Ueffing „
    Differential Protein Profiling of Primary versus Immortalized Human RPE Cells Identifies Expression Patterns Associated with Cytoskeletal Remodeling and Cell Survival
    J Prot. Res. 2006 Apr; 5(4):862-78
11. F Paquet-Durand, S Azadi, SM Hauck, M Ueffing, T van Veen and P Ekstrom.
    Calpain is activated in degenerating photoreceptors in the rd1 mouse.
    J Neurochem. 2006 Feb; 96(3):802-14.
12. SM Hauck, P Ekström, P Ahuja-Jensen, S Suppmann, F Paquet-Durand, T van Veen and M Ueffing.
    Differential Modification of Phosducin Protein in Degenerating rd1 Retina is Associated with Constitutively Active CaMKII in Rod Outer Segments
    Mol Cell Proteomics. 2006 Feb; 5(2):324-36
13. SM Hauck, S Schoeffmann, CA Deeg, CJ Gloeckner, M Swiatek-deLange and M Ueffing
    Proteomic analysis of the porcine interphotoreceptor matrix.
    Proteomics 2005 Sep; 5(14):3623-36.
14. SM Hauck, S Suppmann and M Ueffing
    Proteomic profiling of primary retinal Muller glia cells reveals a shift in expression patterns upon adaptation to in vitro conditions.
    Glia. 2003 Dec; 44(3):251-63.
15. CS Alge, S Suppmann, SG Priglinger, AS Neubauer, CA May, S Hauck, U Welge-Lussen, M Ueffing and A Kampik
    Comparative proteome analysis of native differentiated and cultured dedifferentiated human RPE cells
    Invest Ophthalmol Vis Sci. 2003 Aug; 44(8):3629-41