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François Paquet-Durand

Current Research Projects

Inherited retinal degenerations (e. g. Retinitis Pigmentosa) are a group of blinding diseases that are untreatable at present. I am interested in the mechanisms of photoreceptor cell death during retinal degeneration with the hope that once these processes are fully understood new perspectives for the treatment of during inherited retinal degeneration will open up. To this end I study metabolic processes in degenerating photoreceptors using for instance live cell imaging in combination with in situ biochemical detection of enzymatic activity.

Methodology

  • Neuronal cell culture
  • Organotypic retinal explant culture
  • different in vivo application methods
  • immunofluorescence and - blot
  • in situ biochemical assays
  • live cell imaging
  • deconvolution microscopy

Selected Publications

  1. Paquet-Durand F, Beck S, Michalakis S, Goldmann T, Huber G, Mühlfriedel R, Trifunovic D, Fischer MD, Fahl E, Duetsch G, Becirovic E, Wolfrum U, van Veen T, Biel M, Tanimoto N, Seeliger MW:
    A key role for cyclic–nucleotide gated (CNG) channels in cGMP-related retinitis pigmentosa.
    Hum Mol Gen, 20:941-7, 2011.
  2. Sahaboglu A, Tanimoto N, Kaur J, Sancho-Pelluz J, Huber G, Fahl E, Arango-Gonzalez B, Zrenner E, Ekström P, Löwenheim H, Seeliger MW, Paquet-Durand F:
    PARP1 gene knock-out increases resistance to retinal degeneration without affecting retinal function.
    PLoS One, 5:e15495, 2010.
  3. Paquet-Durand F, Sanges D, McCall J, Silva J, van Veen T, Marigo V, Ekström P:
    Photoreceptor rescue and toxicity induced by different calpain inhibitors.
    J Neurochem, 115:930-40, 2010.
  4. Trifunović D, Dengler K, Michalakis S, Zrenner E, Wissinger B, Paquet-Durand F:
    cGMP-dependent cone photoreceptor degeneration in the cpfl1 mouse retina.
    J Comp Neurol, 518:3604-17, 2010.
  5. Sancho-Pelluz J, Alavi MV, Sahaboglu A, Kustermann S, Farinelli P, Azadi S, van Veen T, Romero FJ, Paquet-Durand F, Ekström P:
    Excessive HDAC activation is critical for neurodegeneration in the rd1 mouse.
    Cell Death Dis, 1: e24, 2010.
  6. Paquet-Durand F, Hauck SM, van Veen T, Ueffing M, Ekström P:
    PKG activity causes photoreceptor cell death in two Retinitis Pigmentosa models.
    J Neurochem, 108:796-810, 2009.
  7. Sancho-Pelluz J, Arango-Gonzalez B, Kustermann S, Romero FJ,  van Veen T, Zrenner E, Ekström P, Paquet-Durand F:
    Photoreceptor cell death mechanisms in inherited retinal degeneration.
    Mol Neurobiol, 38:253-69, 2008.
  8. Paquet-Durand F, Silva J, Talukdar T, Johnson L, Azadi S, Hauck S, Ueffing M, van Veen T, Ekström P:
    Excessive activation of poly (ADP-ribose) polymerase (PARP) contributes to inherited photoreceptor degeneration in the rd1 mouse.
    J Neurosci 27, 10311-10319, 2007.
  9. Paquet-Durand F, Azadi S, Hauck S M, Ueffing M, van Veen T, Ekström P:
    Calpain is activated in degenerating photoreceptors in the rd1 mouse.
    J Neurochem 96, 802-814, 2006.
  10. Hauck SM, Ekström PA, Ahuja-Jensen P, Suppmann S, Paquet-Durand F, van Veen T, Ueffing M:
    Differential modification of phosducin protein in degenerating rd1 retina is associated with constitutively active CaMKII in rod outer segments.
    Mol Cell Proteomics, 5, 324-336, 2006.

 

François Paquet-Durand
François Paquet-Durand

Research Group

Division of Experimental Ophthalmology

Contact

University of Tübingen

Centre for Ophthalmology
Institute for Ophthalmic Research

Roentgenweg 11
72076 Tuebingen
Germany

Phone: +49-7071-29-87430
Fax: +49-7071-29-5777

Email:
francois.paquet-durand[at]klinikum.uni-tuebingen.de

Research Groups

People

Related Research Groups

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nitric oxide
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photoreceptors
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retinal degenerations: hereditary
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retinitis pigmentosa
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second messengers: pharmacology/physiology
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transcription factors
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