You are here: vision-research.eu » People » Research Groups » Germany » Wolfrum, Uwe

Sensory cell biology

The research team is interested retinal cell biology studying the role of the cytoskeleton in respect to the function of sensory cells. We analyze processes and protein complexes associated to the intracellular transport, in particularly the function of centrins and the protein networks (protein interactome) related to the human Usher syndrome.

Our “therapy team” develops gene based therapy strategies for the Usher syndrome for future clinical use. In the lab training of undergraduate and graduate students of the “Neuro Graduate School” of the Johannes Gutenberg University is integrated in the research topics.

Methodology

  • biochemistry, cell and molecular biology techniques (e.g. mRNA/protein expression analyses; protein interaction assays, Y2H system, TAP-tags, Co-IP, GST pull down; organotypic retina culture; transgenic animals).
  • conventional/immunoelectron microscopy, cryotechniques; high resolution fluorescence microscopy; confocal microscopy
  • gene transfer; adeno-associated viruses; nanoparticles; subretinal injections; read through

Scientific Cooperations

  • Cooperation 1
    H. Kremer, R. Roepman (Radboud University Nijmegen, The Netherlands)
  • Cooperation 2
    A. Pulvermüller, P. Hofmann (Charité, Berlin, Germany)
  • Cooperation 3
    M. Ueffing (Helmholtz Zentrum Munich, Germany)
  • Cooperation 4
    W.W. Hauswirth (University of Florida, Gainesville, FL, USA)
  • Cooperation 5
    T. Ben-Yossef, T. Baasov (Technion, Haifa, Israel)

Group Leader

Uwe Wolfrum
[more information]

Contact

Johannes Gutenberg-University Mainz

Cell und Matrix Biology
Institute of Zoology
Muellerweg
655099 Mainz
Germany

Phone: +49 6131 392-5148
Fax: +49 6131 392-3815

Email:
wolfrum[at]uni-mainz.de

Website:
www.ag-wolfrum.bio.uni-mainz.de

Current Research Projects

  • Project 1
    Functions of centrins in retinal photoreceptors (DFG)
  • Project 2
    Molecular transport in photoreceptor cells (DFG)
  • Project 3
    Decipherment of the interactome related to the human Usher syndrome (DFG, FAUN)
  • Project 4
    Gene based therapies for human Usher syndrome (FFB, FAUN):

    • recombinant adeno-associated viruses
    • nanoparticle mediated gene transfer
    • gene repair via homologous recombination mediated by Zink finger nucleases
    • read through of missense mutations mediated by aminoglycosides

Research Groups

People

Related Research Groups

cytoskeleton
Oops, an error occurred! Code: 20191208033915882c17d0
gene transfer/gene therapy
Oops, an error occurred! Code: 201912080339151e3185c5
inner retina dysfunction: biochemistry and cell biology
Oops, an error occurred! Code: 201912080339151ee86f2f
microscopy: electron microscopy
Oops, an error occurred! Code: 20191208033915c0bbb7d3
microscopy: light/fluorescence/immunohistochemistry
Oops, an error occurred! Code: 2019120803391537a280f2
photoreceptors
Oops, an error occurred! Code: 20191208033915444e604b
protein purification and characterization
Oops, an error occurred! Code: 20191208033915d102ad08
protein structure/function
Oops, an error occurred! Code: 201912080339155549825e
proteins encoded by disease genes
Oops, an error occurred! Code: 20191208033915c52d82d9
retinal culture
Oops, an error occurred! Code: 20191208033915665362b5
retinal degenerations: cell biology
Oops, an error occurred! Code: 201912080339152d9c8ad6
retinitis pigmentosa
Oops, an error occurred! Code: 201912080339150190b963
retinal cell biology
Oops, an error occurred! Code: 201912080339152ebd085c
molecular transport
Oops, an error occurred! Code: 20191208033915f55bee05
usher syndrome
Oops, an error occurred! Code: 201912080339150f9ffb72
gene based therapy
Oops, an error occurred! Code: 2019120803391509945fb3